Repositório Institucional da Produção Científica da Marinha do Brasil (RI-MB)

Please use this identifier to cite or link to this item: https://www.repositorio.mar.mil.br/handle/ripcmb/844729
Title: Human norovirus detection in bivalve shell fi sh in Brazil and evaluation of viral infectivity using PMA treatment
Authors: Sarmento, Sylvia Kahwage
Guerra, Caroline Rezende
Malta, Fábio Correia
Coutinho, Ricardo
Miagostovich, Marize Pereira
Fumian, Tulio Machado
Keywords: Ciência, tecnologia e inovação
Ecologia Marinha
Rio de Janeiro
DGPM knowledge areas: Ciência, Tecnologia e Inovação
Issue Date: 2020
Publisher: Escola de Guerra Naval (EGN)
Abstract: Noroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shell fish consumption is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and 2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the consumption of contaminated molluscs.
Access: Restricted access
URI: http://www.repositorio.mar.mil.br/handle/ripcmb/844729
Type: Journal article
Appears in Collections:Ciência, Tecnologia e Inovação: Coleção de Artigos

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