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|Título:||Human norovirus detection in bivalve shell fi sh in Brazil and evaluation of viral infectivity using PMA treatment|
|Autor(es):||Sarmento, Sylvia Kahwage|
Guerra, Caroline Rezende
Malta, Fábio Correia
Miagostovich, Marize Pereira
Fumian, Tulio Machado
|Palavras-chave:||V – CIÊNCIA, TECNOLOGIA E INOVAÇÃO|
V5 - ECOLOGIA MARINHA
RIO DE JANEIRO
|Data do documento:||2020|
|Abstract:||Noroviruses are the most common cause of gastroenteritis outbreaks in humans and bivalve shell fish consumption is a recognized route of infection. Our aim was to detect and characterize norovirus in bivalves from a coastal city of Brazil. Nucleic acid was extracted from the bivalve's digestive tissue concentrates using magnetic beads. From March 2018 to June 2019, 77 samples were screened using quantitative RT-PCR. Noroviruses were detected in 41.5%, with the GII being the most prevalent (37.7%). The highest viral load was 3.5 × 106 and 2.5 × 105 GC/g in oysters and mussels, respectively. PMA-treatment demonstrated that a large fraction of the detected norovirus corresponded to non-infectious particles. Genetic characterization showed the circulation of the GII.2[P16] and GII.4[P4] genotypes. Norovirus detection in bivalves reflects the anthropogenic impact on marine environment and serves as an early warning for the food-borne disease outbreaks resulting from the consumption of contaminated molluscs.|
|Aparece nas coleções:||Ciência, Tecnologia e Inovação: Coleção de Artigos|
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